Details of Award
NERC Reference : NER/T/S/2001/00343
Assessing contributions to fitness of individual genes via genome-wide competition analysis.
Grant Award
- Principal Investigator:
- Professor SG Oliver, The University of Manchester, Life Sciences
- Co-Investigator:
- Professor DB Kell, University of Liverpool, Institute of Integrative Biology
- Co-Investigator:
- Professor GW Griffith, Aberystwyth University, IBERS
- Grant held at:
- The University of Manchester, Life Sciences
- Science Area:
- Terrestrial
- Overall Classification:
- Terrestrial
- ENRIs:
- Pollution and Waste
- Natural Resource Management
- Biodiversity
- Science Topics:
- Environmental Microbiology
- Environmental Genomics
- Population Genetics/Evolution
- Population Ecology
- Abstract:
- In unicellular microbes, fitness = growth rate, and very small changes in growth rate, and thus fitness, may be discriminated using competition analysis in chemostat culture. Molecularly bar-coded strains of bakers yeast containing all possible (Ca 6000) single-gene deletions will be subjected to competition analysis (250 strains at a time) in chemostats limited in 6 different ways. The growth rates and gene expression profiles will be analysed, respectively, by transcriptome arrays (a) newly produced for the bar-codes and (b) those existing under COGEME for every mRNA/cDNA. Subsets will be separated by flow cytometric cell sorting of strains stained for viability and levels of membrane energisation and similarly analysed. Data will be stored in the Manchester Genome Information Management System. Genetic programming methods will be used to provide optimal rules describing the relationship between gene expression levels and fitness in each environment. Other environments chosen will include those containing toxic metals and xenobiotics, oxidative stress, osmotic stress conditions, and the presence of weak acids (such as those used as preservatives for fruit juices). Finally, strains will be studied in their natural environments, in juices, rotting fruit and in oak tree exudates. This unique genetic resource will provide an unparalleled opportunity to assess the global contribution to fitness of every gene in a single organism.
- Period of Award:
- 1 Sep 2002 - 31 Aug 2005
- Value:
- £382,130 Lead Split Award
Authorised funds only
- NERC Reference:
- NER/T/S/2001/00343
- Grant Stage:
- Completed
- Scheme:
- Directed Pre FEC
- Grant Status:
- Closed
- Programme:
- Environmental Genomics
This grant award has a total value of £382,130
FDAB - Financial Details (Award breakdown by headings)
| Total - Staff | Total - T&S | Total - Other Costs | Total - Indirect Costs | Total - Equipment |
|---|---|---|---|---|
| £117,838 | £4,246 | £177,487 | £54,204 | £28,356 |
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