Details of Award
NERC Reference : NER/T/S/2001/00273
The molecular basis of biorhythmicity and environmental signal transduction in marine organisms.
Grant Award
- Principal Investigator:
- Professor PJW Olive, Newcastle University, Marine Science and Technology
- Co-Investigator:
- Professor E Rosato, University of Leicester, Genetics
- Co-Investigator:
- Professor C Kyriacou, University of Leicester, Genetics
- Grant held at:
- Newcastle University, Marine Science and Technology
- Science Area:
- Marine
- Overall Classification:
- Marine
- ENRIs:
- Global Change
- Biodiversity
- Science Topics:
- Environmental Genomics
- Environmental Physiology
- Behavioural Ecology
- Abstract:
- Organisms coordinate with environmental cycles using endogenous clocks but the molecular basis is unknown in marine animals, in stark contrast to model organisms. To address this, RNA from Nereis virens brains, a well characterised marine lophotrochozoan, in multiple phases of circadian and tidal cycles and two reproductive states will be used to construct a normalised cDNA library (approx 1.5+Kb transcripts) and create a microarray. An approx 3+KB cDNA library will be made to screen full length Nereis gene transcripts. The microarray will be probed using CY3/Gy5 straining of reference and competing RNAs to reveal expression profiles for tidal, circadian and photoperiodic treatments. Cycling genes will be sequenced and compared with genomic data. Homology screening , using primers based on known and discovered clock genes, and in situ hybridisation will be carried out and regional adaptation investigated.
- NERC Reference:
- NER/T/S/2001/00273
- Grant Stage:
- Completed
- Scheme:
- Directed Pre FEC
- Grant Status:
- Closed
- Programme:
- Environmental Genomics
This grant award has a total value of £603,060
FDAB - Financial Details (Award breakdown by headings)
| Total - Staff | Total - T&S | Total - Other Costs | Total - Indirect Costs | Total - Equipment |
|---|---|---|---|---|
| £268,250 | £19,740 | £148,866 | £134,204 | £32,000 |
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