Details of Award
NERC Reference : NER/T/S/2001/00253
(Meta)genomic analysis of the nitrogen cycle - a new approach to an old experiment.
Grant Award
- Principal Investigator:
- Professor AWB Johnston, University of East Anglia, Biological Sciences
- Co-Investigator:
- Professor D Richardson, University of East Anglia, Biological Sciences
- Co-Investigator:
- Professor P Hirsch, Rothamsted Research, Sustainable Soils and Crops
- Co-Investigator:
- Dr S Spiro, University of East Anglia, Biological Sciences
- Co-Investigator:
- Dr PL Bond, University of East Anglia, Biological Sciences
- Grant held at:
- University of East Anglia, Biological Sciences
- Science Area:
- Terrestrial
- Overall Classification:
- Terrestrial
- ENRIs:
- Biodiversity
- Science Topics:
- Environmental Microbiology
- Environmental Genomics
- Population Genetics/Evolution
- Population Ecology
- Soil science
- Abstract:
- We will make Metagenomic Libraries, using fragments, ca 30 kb in size, cloned in the wide host-range cosmid vector, pLAFR1. The DNA will be obtained directly from bacteria in soils at the unique Rothamsted Broadbalk Experiment which differ in their N fertiliser regimes over the past 170 years. Libraries will be screened for functional genes for several steps in the N-Cycle by transferring them into appropriate mutants of various bacterial taxa. The resultant genes and flanking sub-genomes will be analysed. Thus, we will identify novel, processes, genes and genomes involved in this important biogeochemical cycle, even in bacteria that cannot be cultured. We will also determine the effects of long-term environmental differences on biodiversity of soil bacteria, at the levels of the genes, the processes, the genomes and the taxa that are responsible for driving the N-cycle.
- NERC Reference:
- NER/T/S/2001/00253
- Grant Stage:
- Completed
- Scheme:
- Directed Pre FEC
- Grant Status:
- Closed
- Programme:
- Environmental Genomics
This grant award has a total value of £352,740
FDAB - Financial Details (Award breakdown by headings)
| Total - T&S | Total - Staff | Total - Other Costs | Total - Equipment | Total - Indirect Costs |
|---|---|---|---|---|
| £5,035 | £158,803 | £79,376 | £36,477 | £73,050 |
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