Details of Award
NERC Reference : NER/T/S/2001/00250
Data mining the Fugu genome to charcterise genes regulating muscle growth and their responses to temperature in a marine fish.
Grant Award
- Principal Investigator:
- Professor IA Johnston, University of St Andrews, Biology
- Co-Investigator:
- Dr J Kinghorn, University of St Andrews, Biology
- Grant held at:
- University of St Andrews, Biology
- Science Area:
- Marine
- Overall Classification:
- Marine
- ENRIs:
- Natural Resource Management
- Global Change
- Biodiversity
- Science Topics:
- Environmental Genomics
- Abstract:
- The phenotypic plasticity of muscle growth will be investigated in relation to temperature in the marine fish Fugu rubripes at the embryo and juvenile stages of the life cycle using an observational and hypothesis testing approach. Bioinformatics techniques will be used to mine sequence information from the genome sequence to identify orthologues of gene loci involved in muscle growth in mammals. Computational analysis will be applied to interrogate gene promoter regions to identify muscle-related cis-acting regulatory sites on a genome-wide basis. As a complimentary approach, subtracted cDNA libraries prepared from embryo/larval and adult stages will be used to identify candidate genes involved in muscle fibre recruitment. The expression patterns of novel and known genes will be investigated using competitive PCR and in situ hybridisation.
- NERC Reference:
- NER/T/S/2001/00250
- Grant Stage:
- Completed
- Scheme:
- Directed Pre FEC
- Grant Status:
- Closed
- Programme:
- Environmental Genomics
This grant award has a total value of £270,187
FDAB - Financial Details (Award breakdown by headings)
| Total - T&S | Total - Staff | Total - Other Costs | Total - Equipment | Total - Indirect Costs |
|---|---|---|---|---|
| £22,449 | £78,787 | £54,682 | £67,148 | £47,119 |
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