Details of Award
NERC Reference : NER/T/S/2000/01347
Biodiversity and molecular probes for in situ identification of gymnamoebae - new tools to allow simple and accurate identification of amoeboid . . .
Grant Award
- Principal Investigator:
- Professor T Cavalier-Smith, University of Oxford, Zoology
- Grant held at:
- University of Oxford, Zoology
- Science Area:
- None
- Overall Classification:
- Freshwater
- ENRIs:
- Pollution and Waste
- Global Change
- Biodiversity
- Science Topics:
- Environmental Microbiology
- Systematics & Taxonomy
- Population Genetics/Evolution
- Community Ecology
- Abstract:
- Naked lobose amoebae (12 families; 4 orders) are a key group in mass and energy flows in aquatic ecosystems. But study of their biodiversity, spatial distribution and roles in food webs is limited because they cannot be reliably identified to species without time-consuming culturing and studies of pure clones. To prepare and test group-specific and species-specific probes for identifying amoebae, we will sequence 18S rRNA genes from all 13 Vanella species and representatives of a more speciose family and all other genera. The probes will be used to identify amoebae by FISH and to compare their biodiversity in DNA samples from Priest Pot (UK), Valamo Lake (Russia) and Niva Bay (Denmark) by PCR and sequencing. To make higher level amoeba classification evolutionarily sounder we shall also sequence and phylogenetically analyse two or three heat-shock proteins from each family.
- NERC Reference:
- NER/T/S/2000/01347
- Grant Stage:
- Completed
- Scheme:
- Directed Pre FEC
- Grant Status:
- Closed
- Programme:
- Marine & Freshwater Microbial
This grant award has a total value of £71,440
FDAB - Financial Details (Award breakdown by headings)
| Total - Staff | Total - T&S | Total - Other Costs | Total - Equipment | Total - Indirect Costs |
|---|---|---|---|---|
| £19,736 | £20,585 | £13,515 | £8,526 | £9,077 |
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