Details of Award
NERC Reference : NER/M/S/2003/00097
Comparison of methods used to analyse microbial communities in situ.
Grant Award
- Principal Investigator:
- Dr K Purdy, University of Reading, Animal and Microbial Sciences
- Grant held at:
- University of Reading, Animal and Microbial Sciences
- Science Area:
- Marine
- Freshwater
- Overall Classification:
- Marine
- ENRIs:
- Biodiversity
- Science Topics:
- Environmental Microbiology
- Biogeochemical Cycles
- Abstract:
- Understanding the structure and function of microbial communities is the fundamental process in microbial ecology. However, the determination of the population size and activity of microbial communities in the environment is extremely difficult. This proposal seeks to contrast and compare three methods that are commonly used to analyse microbial communities in situ in order to assess their relative merits. These will be oligonucleotide probing of extracted DNA, Fluorescent In Situ Hybridisation and Real-Time PCR. The accuracy, precision and detection limits of these methods will be determined using an artificial mixture of target and non-target organisms as well as samples taken from the estuarine sediments of the River Colne in Essex. Overall costs, including lab-time and ease of use will be assessed in the final analysis and the best method determined.
- NERC Reference:
- NER/M/S/2003/00097
- Grant Stage:
- Completed
- Scheme:
- New Investigators Pre FEC
- Grant Status:
- Closed
- Programme:
- New Investigators
This grant award has a total value of £51,409
FDAB - Financial Details (Award breakdown by headings)
| Total - Staff | Total - T&S | Total - Other Costs | Total - Indirect Costs | Total - Equipment |
|---|---|---|---|---|
| £27,327 | £211 | £9,113 | £12,585 | £2,174 |
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