Details of Award
NERC Reference : NER/B/S/2000/00730
The distribution of Listeria sp. in the environment, a study investigating the role of soil protozoans in their life history.
Grant Award
- Principal Investigator:
- Professor EMH Wellington, University of Warwick, Biological Sciences
- Grant held at:
- University of Warwick, Biological Sciences
- Science Area:
- None
- Overall Classification:
- Terrestrial
- ENRIs:
- Pollution and Waste
- Environmental Risks and Hazards
- Biodiversity
- Science Topics:
- Environmental Microbiology
- Population Ecology
- Community Ecology
- Soil science
- Abstract:
- We aim to determine to what extent the bacterial pathogens Listeria species can colonise protozoans and survive intracellular digestion. Colonisation of protozoa may provide an ideal habitat for listeria during the part of their life cycle outside of the host. Listeria can colonise a wide range of vertebrate and invertebrate hosts but little is known of their ecology. The main objectives are to screen community DNAs from a range of habitats with listeria-specific PCR primers to enable detection and select those samples giving PCR products. These will be used for extraction of protozoans which will be screened for intracellular presence of listeria. In addition a lab-based study will investigate the potential for intracellular survival in co-cultures of Acanthamoeba polypaga and Listeria monocytogenes using gfp reporter constructs for virulence determinants.
- NERC Reference:
- NER/B/S/2000/00730
- Grant Stage:
- Completed
- Scheme:
- Small Grants Pre FEC
- Grant Status:
- Closed
- Programme:
- Small Grants
This grant award has a total value of £26,890
FDAB - Financial Details (Award breakdown by headings)
| Total - Staff | Total - Other Costs | Total - Indirect Costs |
|---|---|---|
| £14,290 | £6,027 | £6,573 |
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