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Details of Award

NERC Reference : NER/A/S/2001/01097

Cloud borne bacteria: community composition and ice nucleating ability.

Grant Award

Principal Investigator:
Dr B Moffett, University of East London, Health, Sport and Biosciences
Co-Investigator:
Professor D Fowler, UK Centre for Ecology & Hydrology, Biodiversity (Penicuik)
Co-Investigator:
Professor TW Choularton, The University of Manchester, Earth Atmospheric and Env Sciences
Science Area:
Atmospheric
Overall Classification:
Atmospheric
ENRIs:
Biodiversity
Science Topics:
Environmental Microbiology
Boundary Layer Meteorology
Technol. for Environ. Appl.
Abstract:
We aim to analyse bacterial communities in orographic clouds and a cloud water sample collected from an aircraft. Seasonal variability in community structure will be determined using terminal restriction fragment length polymorphism (TRFLP) analysis. A full description of the community will be obtained using amplified ribosomal DNA restriction analysis (ARDRA) with targeted sequencing. The feasibility of using reverse transcriptase PCR to investigate the presence of a metabolically active bacterial fraction will be assessed. An important functional attribute, ice nucleating ability (INA), will also be investigated in 3 ways - in vitro measurement of the freezing properties of cloud water: the effect cloud bacterial isolates have on the freezing properties of water: and the detection of ice nucleating genes by PCR.
Period of Award:
1 Aug 2002 - 31 Oct 2004
Value:
£132,634
Authorised funds only
NERC Reference:
NER/A/S/2001/01097
Grant Stage:
Completed
Scheme:
Standard Grants Pre FEC
Grant Status:
Closed
Programme:
Standard Grant

This grant award has a total value of £132,634  

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FDAB - Financial Details (Award breakdown by headings)

Total - T&STotal - StaffTotal - Other CostsTotal - Indirect Costs
£4,108£75,944£17,649£34,935

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