Details of Award
NERC Reference : NER/A/S/2001/01097
Cloud borne bacteria: community composition and ice nucleating ability.
Grant Award
- Principal Investigator:
- Dr B Moffett, University of East London, Health, Sport and Biosciences
- Co-Investigator:
- Professor D Fowler, UK Centre for Ecology & Hydrology, Biodiversity (Penicuik)
- Co-Investigator:
- Professor TW Choularton, The University of Manchester, Earth Atmospheric and Env Sciences
- Grant held at:
- University of East London, Health, Sport and Biosciences
- Science Area:
- Atmospheric
- Overall Classification:
- Atmospheric
- ENRIs:
- Biodiversity
- Science Topics:
- Environmental Microbiology
- Boundary Layer Meteorology
- Technol. for Environ. Appl.
- Abstract:
- We aim to analyse bacterial communities in orographic clouds and a cloud water sample collected from an aircraft. Seasonal variability in community structure will be determined using terminal restriction fragment length polymorphism (TRFLP) analysis. A full description of the community will be obtained using amplified ribosomal DNA restriction analysis (ARDRA) with targeted sequencing. The feasibility of using reverse transcriptase PCR to investigate the presence of a metabolically active bacterial fraction will be assessed. An important functional attribute, ice nucleating ability (INA), will also be investigated in 3 ways - in vitro measurement of the freezing properties of cloud water: the effect cloud bacterial isolates have on the freezing properties of water: and the detection of ice nucleating genes by PCR.
- NERC Reference:
- NER/A/S/2001/01097
- Grant Stage:
- Completed
- Scheme:
- Standard Grants Pre FEC
- Grant Status:
- Closed
- Programme:
- Standard Grant
This grant award has a total value of £132,634
FDAB - Financial Details (Award breakdown by headings)
Total - T&S | Total - Staff | Total - Other Costs | Total - Indirect Costs |
---|---|---|---|
£4,108 | £75,944 | £17,649 | £34,935 |
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