Details of Award
NERC Reference : NER/A/S/2000/01253
Impact of pollu on dissem of bacterial genes (qac) encoding resistance to quaternary ammonium compounds & evid for co-selec of drug resistance genes
Grant Award
- Principal Investigator:
- Professor EMH Wellington, University of Warwick, Biological Sciences
- Co-Investigator:
- Professor PM Hawkey, University of Birmingham, Institute of Microbiology and Infection
- Grant held at:
- University of Warwick, Biological Sciences
- Science Area:
- Terrestrial
- Freshwater
- Overall Classification:
- Freshwater
- ENRIs:
- Pollution and Waste
- Environmental Risks and Hazards
- Science Topics:
- Environmental Microbiology
- Ecotoxicology
- Community Ecology
- Technol. for Environ. Appl.
- Abstract:
- A survey of bacterial phenotypic resistance to surface-active quaternary ammonium compounds (QACs) will be done at six sites representing different levels of QAC pollution including textile mill effluent and sewage sludge. QAC levels will be monitored in relation to occurrence of qac genotypes, isolation media will target specific groups, and isolates characterised for diversity and presence of qac genes. A biosensor strain will be developed to assess bioavailable QAC levels in situ. Transferable resistance will be determined by exogenous isolation using seeded marked hosts, Direct community analysis will be done using degenerate sets of qac--specific PCR primers. Community DNA will also be screened for antibiotic resistance genes. Evidence for co--selection of antibiotic resistance will be sort by PCR screening and sensitivity testing of isolates.
- NERC Reference:
- NER/A/S/2000/01253
- Grant Stage:
- Completed
- Scheme:
- Standard Grants Pre FEC
- Grant Status:
- Closed
- Programme:
- Standard Grant
This grant award has a total value of £200,454
FDAB - Financial Details (Award breakdown by headings)
| Total - T&S | Total - Staff | Total - Other Costs | Total - Indirect Costs | Total - Equipment |
|---|---|---|---|---|
| £2,322 | £89,913 | £38,790 | £41,360 | £28,070 |
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