Details of Award
NERC Reference : NE/S008721/1
LinkPI: Linking Phenotype function with Identity: a novel integrated single-cell technology and metagenomics approach
Grant Award
- Principal Investigator:
- Professor H Yin, University of Glasgow, School of Engineering
- Co-Investigator:
- Professor EMH Wellington, University of Warwick, School of Life Sciences
- Co-Investigator:
- Professor W Huang, University of Oxford, Engineering Science
- Co-Investigator:
- Prof. C Quince, Earlham Institute, Research Faculty
- Grant held at:
- University of Glasgow, School of Engineering
- Science Area:
- Freshwater
- Terrestrial
- Overall Classification:
- Unknown
- ENRIs:
- Biodiversity
- Environmental Risks and Hazards
- Pollution and Waste
- Science Topics:
- Microsystems
- Bacterial genomics
- Environmental Genomics
- Bionanotechnology
- Environmental biotechnology
- Microbial diversity
- Soil contamination
- Waste
- Water waste
- Tools for the biosciences
- Abstract:
- The wide ranging and diverse microorganisms found within the environment play a central role in maintaining sustainability on our planet. However, their ability to function and the functions themselves have been and are being seriously altered by human activities. One key example of this is the development of antimicrobial resistance in wild bacteria as a result of antibiotics that can be found in waste water. The impact of this is a significant emerging threat to the global economy and health. Increasing evidence shows that variations in the genetic makeup of individual cells, together with the way these are manifest in their physical characteristics plays a critical role in the fate of these microorganisms. Despite this knowledge, most studies of microorganisms currently rely on culturing and analysing them as large groups in laboratories, rather than on the individual level. To further complicate matters, the majority of the naturally occurring microbes (>99%) are not capable of being grown in laboratory conditions. This imposes formidable challenges to understand the activities of microbes in situ and their response to the ever-changing environments. In this project we will establish a novel approach to identify active microbes of interest within complex microbial communities, linking the behaviour and genetic profile of individual cells. Specifically, we will exploit the cutting-edge technology advances to analyse, sort and characterise microbes from a mixture of microorganisms. To achieve this, we will work to develop new devices and protocols for collecting samples on-site. These samples will then be analysed using a range of cutting-edge, lab-based techniques. One key feature of the project is the forging of new international collaborations with the world leading research groups of complementary expertise. This not only provides us with access to a range of world-class tools, but also enables the local collection and handling of samples from sites of interest around the globe. These include the Yanzi river near Shanghai in China, the River Thames near London and the Han River near Seoul in Korea.
- NERC Reference:
- NE/S008721/1
- Grant Stage:
- Completed
- Scheme:
- Directed - International
- Grant Status:
- Closed
- Programme:
- GPSF
This grant award has a total value of £83,396
FDAB - Financial Details (Award breakdown by headings)
| DI - Other Costs | Indirect - Indirect Costs | DA - Investigators | DA - Estate Costs | DI - T&S | DA - Other Directly Allocated | Exception - T&S |
|---|---|---|---|---|---|---|
| £15,385 | £9,028 | £16,651 | £2,324 | £24,470 | £354 | £15,183 |
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