Details of Award
NERC Reference : NE/N02026X/1
Occurrence and horizontal gene transfer of carbapenemase and ESBL genes in soil microbiomes
Grant Award
- Principal Investigator:
- Professor C Keevil, University of Southampton, Centre for Biological Sciences
- Co-Investigator:
- Dr M G Dumont, University of Southampton, Sch of Biological Sciences
- Grant held at:
- University of Southampton, Centre for Biological Sciences
- Science Area:
- Terrestrial
- Overall Classification:
- Unknown
- ENRIs:
- Biodiversity
- Environmental Risks and Hazards
- Pollution and Waste
- Science Topics:
- Soil microbiology
- Soil science
- Microbial communities
- Environmental Microbiology
- Health risk
- Antibiotic resistance
- DNA sequencing
- Antibacterials
- Microbiology
- Microbiology
- Environmental Genomics
- Microbial communities
- Soil science
- Abstract:
- This project aims to understand the potential of environmental reservoirs and transmission of AMR in members of autochthonous soil microbiomes and between transient allochthonous human and animal pathogens entering the environment, for example from faecal agricultural wastes or domesticated or wild animal and bird faecal ingress. It also seeks to understand if horizontal gene transfer occurs successfully between potential pathogens in the various soil microbiomes and complex matrices encompassing clay, loam and sandy soils. The work will utilize modern molecular biology and genomics to screen microbiome populations and defined third generation ESBL and fourth generation carbapenemase E. coli and Klebsiella pneumoniae donor strains, with an E. coli sensitive strain as the recipient. The University of Southampton team comprises an established scientific group with expertise in survival of faecal pathogens in soil and water, and HGT of ESBL and carbapenemase resistance genes between different species, in collaboration with an Early Career Researcher with expertise in soil microbiology and processes, and stable isotope probing (SIP) to understand community viability, metabolic turnover and gene acquisition. Previous work for UKWIR and Defra/FSA has developed grassed soil microcosms of clay, loam and sandy soils and demonstrated survival of zoonotic pathogens therein for several weeks following faecal waste irrigation. These pathogens were subsequently released from the complex soil matrices using a novel, gentle pulsification procedure followed by membrane filtration and quantitative resuscitation on appropriate selective agar media. This proposal will help identify the specific environmental drivers of the HGT and beta lactamase selection processes, including implications for both anthropogenic (animal husbandry, human wastewater disposal) and non-anthropogenic (wild animal and bird faecal ingress) drivers. The work will also include identifying the implications for pathogens of clinical and/or veterinary importance such Klebsiella pneumonia and E. coli. The research will be able to help inform AMR policy and management strategies, and will form the basis of a subsequent large research proposal to address these policies and strategies more fully. The approaches and methodologies to be developed in this proposal could be translated to other environments, antibacterials or other bacterial communities of interest to the AMR funding bodies.
- NERC Reference:
- NE/N02026X/1
- Grant Stage:
- Completed
- Scheme:
- Directed (Research Programmes)
- Grant Status:
- Closed
- Programme:
- AMR
This grant award has a total value of £158,384
FDAB - Financial Details (Award breakdown by headings)
| DI - Other Costs | Indirect - Indirect Costs | DA - Investigators | DI - Staff | DA - Estate Costs | DA - Other Directly Allocated |
|---|---|---|---|---|---|
| £16,186 | £56,934 | £13,819 | £51,839 | £16,919 | £2,686 |
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