Details of Award
NERC Reference : NE/G020256/1
Evolutionary dynamics of genome obesity
Grant Award
- Principal Investigator:
- Professor AR Leitch, Queen Mary University of London, Sch of Biological and Chemical Sciences
- Co-Investigator:
- Professor RA Nichols, Queen Mary University of London, Sch of Biological & Behavioural Sciences
- Science Area:
- Terrestrial
- Overall Classification:
- Terrestrial
- ENRIs:
- Biodiversity
- Science Topics:
- Environmental Genomics
- Abstract:
- The central dogma in genetics is that 'DNA codes for RNA which makes proteins'. Why then do similar and closely related organisms often have widely different amounts of DNA in their nuclei? Even in organisms with a small amount of DNA, the fraction that comprises genes is frequently very small. What is the extra DNA doing and how did it get there? These are important questions because we know that organisms with large genomes are at greater risk of extinction, are less adaptable to living in polluted soils, and are less able to tolerate extreme environmental conditions. Clearly the total amount of DNA has ecological consequences which shape the distribution and persistence of biodiversity. In fact an analysis of many thousands of species reveals that genome size varies enormously. In plants alone it can vary nearly 2000-fold. The non-genic component of DNA is usually composed of highly repeated sequences of two types, (i) dispersed repetitive sequences and (ii) tandem repeated sequences. Together these give the chromosomes a particular character, such as their size. Chromosome size is important in the evolution and diversification of all organisms because large chromosomes (or more accurately the total sum of all chromosomes = the genome) often come at a cost, including long generation times, slow development and restrictions on the type of habitat occupied by the organism. There may nevertheless be advantages to having large genomes in some circumstances because some organisms do have particularly large amounts of DNA. In the last 20 years there has been an enormous effort expended by the science community to increase our understanding of plant genomes for three main reasons: (i) to improve the efficiency, versatility and value of agriculture; (ii) to harness resources for medical research, and (iii) to stimulate new discoveries through fundamental research. To achieve this end several plants were selected to have their genomes completely sequenced, so that we could discover in detail the nature and occurrence of genic and non-genic DNA. This was an expensive and time-consuming task involving labs from around the world. To minimise the scale of the task, plants with small genomes, like the weed Arabidopsis thaliana and the crop rice, were selected for analysis. Our current thinking is that over many thousands or millions of years, DNA in the genome can expand through amplification of some repetitive DNA and shrink through small bite-like loses. Nevertheless, this fascinating view of the dynamic nature of plant genomic DNA is flawed because we only understand the dynamics of plants with small to medium-sized genomes, i.e. those genomes chosen for DNA sequencing. Is this picture of genome evolution true for organisms with large genomes? To address this we have selected the plant genus Fritillaria for analysis, since it includes species with truly giant genomes including the largest so far reported for any plant. The problem is how to tackle the Herculean task of determining the nature and evolution of so much DNA in Fritillaria. Fortunately a powerful new method to sequence huge amounts of DNA cost-effectively has been developed that enables us to get a handle on genome evolution in organisms with giant genomes. These methods use sophisticated DNA handling and analytical approaches for studying DNA. When coupled with microscopical studies of the chromosomes themselves, and a detailed understanding of how the species are related to each other, we can build a picture of the evolutionary events that occurred in the formation of giant genomes. We will address what DNA sequences are involved in genome enlargement, why particular sequences became so abundant, and if genome enlargement happened suddenly in evolution, or slowly over time. Thus our study will provide the community with fundamental knowledge of the processes occurring in plant genome evolution.
- Period of Award:
- 7 Feb 2010 - 6 Feb 2013
- Value:
- £193,318 Split Award
Authorised funds only
- NERC Reference:
- NE/G020256/1
- Grant Stage:
- Completed
- Scheme:
- Standard Grant (FEC)
- Grant Status:
- Closed
- Programme:
- Standard Grant
This grant award has a total value of £193,318
FDAB - Financial Details (Award breakdown by headings)
| DI - Other Costs | Indirect - Indirect Costs | DA - Investigators | DI - Staff | DA - Estate Costs | DI - T&S |
|---|---|---|---|---|---|
| £4,927 | £68,968 | £55,425 | £49,082 | £10,728 | £4,188 |
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