Details of Award
NERC Reference : NER/A/S/2001/01130
How do marine diatoms fix ten billion tonnes of inorganic carbon per year?
Grant Award
- Principal Investigator:
- Professor RC Leegood, University of Sheffield, Animal and Plant Sciences
- Co-Investigator:
- Professor J Raven, University of Dundee, School of Life Sciences
- Grant held at:
- University of Sheffield, Animal and Plant Sciences
- Science Area:
- Marine
- Overall Classification:
- Marine
- ENRIs:
- Natural Resource Management
- Global Change
- Science Topics:
- Environmental Microbiology
- Environmental Physiology
- Climate & Climate Change
- Abstract:
- Diatoms are responsible for at least a quarter of the greater than 4. 10 15 g (four peta grams) inorganic carbon fixed each year in the ocean. The aim of this work is to investigate the claim that marine diatoms, such as Thalassiosira weissflogii, possess C4 photosynthesis and to establish the existence and roles of PEP carboxylase and PEP carboxykinase. We will investigate (i) which 'C3 + C1' carboxylases are present and their intracellular location. This will be done by purifying PEP carboxylase and PCK from T weissflogii and raising antibodies against both enzymes, (ii) C4 metabolism by tracing the labelling of metabolites by 13CO2 using mass-spectrometry, (iii) the role(s) of PEP carboxylase and PCK in C4 and/or anaplerotic metabolism. We will manipulate the balance between photosynthesis and anaplerosis during growth by changing light, CO2 and N and measure the amounts of enzymes and patterns of metabolism.
- Period of Award:
- 24 Feb 2003 - 23 Feb 2006
- Value:
- £147,823 Lead Split Award
Authorised funds only
- NERC Reference:
- NER/A/S/2001/01130
- Grant Stage:
- Completed
- Scheme:
- Standard Grants Pre FEC
- Grant Status:
- Closed
- Programme:
- Standard Grant
This grant award has a total value of £147,823
FDAB - Financial Details (Award breakdown by headings)
| Total - T&S | Total - Staff | Total - Other Costs | Total - Equipment | Total - Indirect Costs |
|---|---|---|---|---|
| £5,493 | £79,314 | £23,875 | £2,657 | £36,484 |
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